师资队伍

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杨荣华教授

发布日期: 2015-03-12 来源:

 

   分析化学,博士,教授、博士生导师。“长江学者”特聘教授、国家杰出青年科学基金获得者。长沙理工大学化学与生物工程学院院长。
电话:
0731-85258724 88822523   电子信箱: Yangrh@pku.edu.cnYangrh@hnu.edu.cn,

 

   

 

个人简介

1994年湖南大学获分析化学硕士学位,2000年湖南大学获分析化学博士学位。2001-2003年,北京大学博士后;2003-2008年,北京大学副教授、博士生导师。其间, 2006-2007年,(美国)佛罗里达大学访问教授。20089月至今,湖南大学教授、博士生导师。201410月至今,长沙理工大学教授、博士生导师。主要从事化学与生物传感技术研究。主持承担国家自然科学基金委杰出青年科学基金、重点项目、国家重大科学研究计划及湖南省社会发展计划重点项目等多项科研项目。在Acc  Chem  Res、Chem Soc Rev.、Angew Chem Int EdJACSACS NanoChem CommunAnal Chem等期刊发表论文100余篇。获得湖南省科技进步一等奖中国分析测试协会科学技术奖(CAIA)一等奖教育部自然科学一等奖国家自然科学二等奖等奖励。

主讲课程:
    现代分析技术》(博士生)、《化学前沿》、《本科生科研方法训练

主要研究领域】:
 1. 荧光探针设计、合成及生物传感应用

 2. 荧光传感信号转换与放大新原理与新方法
 
3.化学传感器在食品安全与环境分析中的应用
4.
纳米与分子水平上的生命分析化学

代表性论文

[1]    Jing Zheng, Ronghua Yang,* Muling Shi, Cuichen Wu, Xiaohong Fang, Yinhui Li, Jishan Li and Weihong Tan* “Rational designed molecular beacons for bioanalytical and biomedical applications” Chem Soc Rev 2015, DOI:10.1039/c5cs00020c  

[2]    Jing Zheng, Junhui Bai, Qifeng Zhou, Jishan Li, Yinhui Li, Cuichen Wu, Jinfeng Yang and Ronghua Yang* “DNA-templated in situ growth AgNPs on SWNT: A new highly sensitive SERS assay for MicroRNA” Chem. Commun 2015,  DOI 10.1039/C5CC01003A

[3]    Changhui Liu, Zhihe Qing,  Jing Zheng, Li Deng, Cheng Ma, Jishan Li, Yinhui Li, Sheng Yang, Jinfeng Yang, Jing Wang, Weihong Tan, and Ronghua Yang* “DNA-template in situ growth of metal nanoparticles on mesoporous silica nanospheres for intracellular GSH-controlled drug delivery” Chem. Commun2015, DOI:10.1039/c5cc00557d

[4]    Muling Shi, Jing Zheng, Yongjun Tan, Guixiang Tan, Jishan Li, Yinhui Li, Xia Li, Zhiguang Zhou, and Ronghua Yang* “Ultrasensitive detection of single nucleotide polymorphism in human mitochondrial DNA utilizing ion-mediated cascade SERS amplification” Anal Chem 2015, DOI: 10.1021/ac504000p

[5]    Zhehua Zhang, Changhui Liu, Junhui Bai, Cuichen Wu,  Yue Xiao, Yinhui Li, Jing Zheng,* Ronghua Yang,* and Weihong Tan “Silver nanoparticle-gated mesoporous silica-coated gold nanorods (AuNR@MS@AgNPs): Low premature release and multifunctional cancer theranostic platform” ACS Appl. Mater. Interfaces.2015, DOI:10.1021/acsami.5b00368

[6]    Li Deng, Yan Li, Xiuping Yan, Jun Xiao, Cheng Ma, Jing Zheng, Shaojun Liu, and Ronghua Yang* “Ultrasensitive and highly selective detection of bio-accumulation of methyl-mercury in fish samples via Ag0/Hg0 amalgamation” Anal Chem 2015, 87, 2452–2458.

[7]    Yinhui Li, Yijun Wang, Yirong Zhao, Sheng Yang, Lin Yuan, Jing Zheng, Ronghua Yang* Hemicyanine-based high resolution ratiometric near-infrared fluorescent probe for monitoring pH changes in vivo. Anal Chem 2015,  87, 2495–2503.

[8]    Yinhui Li, Yirong Zhao, Winghong Chan, Yijun Wang, Qihua You, Changhui Liu, Jing Zheng Jishan Li, Sheng Yang, Ronghua Yang* “Selective tracking of lysosomal Cu2+ ions using simultaneous target- and location activated fluorescent nanoprobe” Anal Chem  2015, 87, 584–591.

[9]    Lili Wang, Jing Zheng, Yinhui Li, Sheng Yang, Changhui Liu, Yue Xiao, Jishan Li, Zhong Cao, Ronghua Yang* “AgNP-DNA@GQDs hybrid: A new approach for sensitive detection of H2O2 and glucose via simultaneous AgNP etching and DNA cleavage” Anal Chem  2014, 86, 12348–12354.

[10] Huijuan Yan, Leiliang He, Wenjie Zhao, Jishan Li*, Yue Xiao, Ronghua Yang*, and Weihong Tan. “Poly b-cyclodextrin/TPdye nanomicell-based two-photon nanoprobe for caspase-3 activation imaging in live cells and tissues Anal Chem   2014, 86, 11440–11450.

[11] Jing Zheng, Yuhong Nie, Sheng Yang, Yue Xiao, Jishan Li, Yinhui Li, Ronghua Yang* Remote-controlled release of DNA in living cells via simultaneous light and host-guest mediations”. Anal Chem   2014, 86, 10208–10214.

[12] Sheng Yang, Changyao Wang, Changhui Liu, Yijun Wang, Yue Xiao, Jishan Li, Yinhui Li, and Ronghua Yang,* “Fluorescence modulation by absorbent on solid surface: An improved approach for designing fluorescent sensor”  Anal. Chem. 2014, 86 , 7931-7938

[13] Sheng Yang, Yue Qi, Changhui Liu, Yijun Wang, Yirong Zhao, Lili Wang, Jishan Li, Weihong Tan, and Ronghua Yang,* “Design of simultaneous target and location-activatable fluorescent probe for visualizing  hydrogen sulfide in lysosomes”.  Anal Chem 2014, 86, 7508-7515.

[14] Cheng Ma, Tong Bian, Sheng Yang, Changhui Liu, Tierui Zhang, Jinfeng Yang, Yinhui Li, Jishan Li,* Ronghua Yang, and Weihong Tan. “Fabrication of versatile cyclodextrin-functionalized upconversion luminescence nanoplatform for biomedical imaging”.  Anal Chem 2014, 86, 6508-6515.

[15] Qiao Tang, Qier Zhang, Ying Jiang, Jishan Li,* Jing Zheng, Yinhui Li, Ronghua Yang, and Weihong Tan.  Competitive assembly to increase the performance of the DNA/carbon-nanomaterial-based sensing platform. ACS Appl. Mater. Interfaces20146, 13470–13477.

[16] Huijuan Yan, Leiliang He, Cheng Ma, Jishan Li,* Jinfeng Yang, Ronghua Yang* and Weihong Tan. “Poly β-cyclodextrin inclusion-induced formation of two photon imaging.” Chem. Commun., 2014, 50, 8398-8401. 

[17] Zhao Li, Xinyuan He, Zhe Wang, RonghuaYang, Wen Shi, Huimin Ma,* ‘In vivo imaging and detection of nitroreductase in zebrafish by anew near-infrared fluorescence off-on probe” Biosens Bioelectron, 2015, 63, 112-116.

[18] Jia Tao, Yinhui Li, Peng Zhao, Yu Duan, Jishan Li, Wenjie Zhao, Ronghua Yang.* “Development of spiropyran-based electrochemical sensor via simultaneous photochemical and target-activatable electron transfer biosensors and bioelectronics.” Biosens Bioelectron. 2014, 62, 151-157.

[19] Changyao Wang, Sheng Yang, Mei Yi, Changhui Liu, Yijun Wang, Jinhua Liu, Jishan Li, Yinhui Li, and Ronghua Yang* , “Graphene oxide assisted fluorescent chemodosimeter for high-performance sensing and bioimaging of fluoride ions” ACS Appl. Mater. Interfaces, 2014, 6, 9768-9775.

[20] Hao Liang, Xiaobing Zhang*, Yifan Lv, Liang Gong, Ruowen Wang, Xiaoyan Zhu, Ronghua Yang*, and Weihong Tan*, “Functional DNA-containing nanomaterials: Cellular applications in biosensing, imaging, and targeted therapy” Acc. Chem. Res., 2014, 47, 1891-1901.

[21] Mei Yi, Sheng Yang, Zanying Peng, Changhui Liu, Jishan Li*, Wenwan Zhong, Ronghua Yang, and Weihong Tan “Two-photon graphene oxide/aptamer nanosensing conjugate for in vitro or in vivo molecular probing” Anal. Chem. 2014, 86, 3548-3554.

[22] Jing Zheng, Yaping Hu, Junhui Bai, Cheng Ma, Jishan Li, Yinhui Li, Muling Shi, Weihong Tan, Ronghua Yang*, “A universal SERS amplification detector for ultrasensitive detection of multiple target analytes” Anal. Chem. 2014, 86, 2205-2212.

[23] Yinhui Li, Yu Duan, Jing Zheng, Jishan Li, Wenjie Zhao, Sheng Yang, Ronghua Yang* Self-assembly of   graphene oxide with a silyl-appended spiropyran dye for rapid and sensitive colorimetric detection of fluoride ions” Anal. Chem. 2013, 85, 11456–11463.

[24] Li Deng, Xiangyuan Ouyang, Jianyu Jin, Cheng Ma, Ying Jiang, Jing Zheng, Jishan Li, Yinhui Li, Weihong Tan, Ronghua Yang*. “Exploiting the higher specificity of silver amalgamation: selective detection of mercury (II) by forming Ag/Hg amalgam.” Anal. Chem. 2013, 85, 8594-8600. 

[25] Jing Zheng, Guizhi Zhu, Yinhui Li, Chunmei Li,  Mingxu You, Tao Chen, Erqun Song, Ronghua Yang*, and Weihong Tan*.  “A  spherical nucleic acids platform based on self-assembled DNA biopolymer for high performance cancer therapy.” ACS Nano 2013, 7, 6545–6554.  

[26] Juanping Li, Wenyu Zhou, Yuhua Jia, Changhui Liu, Jing Zheng, Yinhui Li, Jishan Li*,  and Ronghua Yang*. “A gold nanocarrier and DNA-metal ligation-based sensing ensemble for fluorescent assay of thiol–containing amino acids and peptides.” Chem. Commun. 2013, 49, 7932—7934.   

[27] Jing Zheng,Yuhong Ni, Yaping Hu, Jishan Li,Yinhui Li, Ying Jiang, Ronghua Yang* .“Time-resolved fluorescent detection of Hg2+ in a complex environment by conjugating magnetic nanoparticles with a triple-helix molecular switch.” Chem Commun. 2013, 49, 6915 – 6917.

[28] Xiaowei Mao, Haiyan Su, Demei Tian, Haibing Li,* Ronghua Yang. “Bipyrene-functionalized graphene as a “Turn-On” fluorescence sensor for manganese(II) ions in living cells”  ACS Appl. Mater. Interfaces 2013, 5, 592597.

[29] Jishan Li,* Yuhua Jia, Jing Zheng, Wenwan Zhong, Guoli Shen, Ronghua Yang,* Weihong Tan. “Aptamer degradation inhibition combined with DNAzyme cascade-based signal amplification for colorimetric detection of proteins”. Chem Commun. 2013, 49, 6137-6139.

[30] Jinhua Liu, Changyao Wang, Ying Jiang, Yaping Hu, Jishan Li, Sheng Yang, Yinhui Li, Ronghua Yang,* Weihong Tan. “Graphene signal amplification for sensitive and real-time fluorescence anisotropy detection of small molecule” Anal. Chem. 2013, 85, 1424-1430.  

[31] Jing Zheng, Anli Jiao, Ronghua Yang,* Huimin Li, Jishan Li, Muling Shi, Cheng Ma, Ying Jiang, Li Deng, Weihong Tan,* “Fabricating a reversible and regenerable Raman-active substrate with a biomolecule-controlled DNA nanomachine” J. Am. Chem. Soc. 2012, 134, 19957–19960.

[32] Yinhui Li, Yu Duan, Jishan Li, Jing Zheng, Huan Yu, Ronghua Yang*. “Simultaneous nucleophilic-substituted and electrostatic interactions for thermal switching of spiropyran: A new approach for rapid and sensitive detection of thiol-containing amino acids using a spiropyran probe” Anal. Chem. 2012, 84. 47324738.

[33] Anli Jiao, Jing Zheng, Yaping Hu, Guizhi Zhu, Jishan Li,* Huimin Li*, Ronghua Yang*, and Weihong Tan. “Hybridization-triggered isothermal signal amplification coupled with MutS for label-free and sensitive fluorescent assay of SNPs.” Chem Commun. 2012, 48, 5659 – 5661.

[34] Xiangyuan Ouyang, Jinhua Liu, Jishan Li*, Ronghua Yang*. “Carbon nanoparticle-based low-background biosensing platform for sensitive and label-free fluorescent assay of DNA methylation” Chem Commun. 2012, 48, 88 – 90.

[35] Jinhua Liu, Jishan Li, Ying Jiang, Sheng Yang, Weihong Tan, Ronghua Yang.* “Combination of p-p stacking and electrostatic repulsion between carboxylic carbon nanoparticles and fluorescent oligonucleotides for rapid and sensitive detection of thrombin” Chem Commun. 2011, 47, 11321-11323.

[36] Jing Zheng, Jishan Li, Ying Jiang, Jianyu Jin, Kemin Wang, Ronghua Yang,*  Weihong Tan. “Design aptamer-based sensing platform using triple-helix molecular switch” Anal. Chem. 2011, 83, 6586–6592 . 

[37] Yunyao Xu, Li Deng, Hao Wang, Xiangyuan Ouyang, Jing Zheng, Jishan Li,* Ronghua Yang* “Metal-induced aggregation of mononucleotides-stabilized gold nanoparticles: An efficient approach for simple and rapid colorimetric detection of Hg(II)”. Chem Commun. 2011, 47, 6039 – 6041.

[38] Jishan Li, Wenyu Zhou, Xiangyuan Ouyang, Huan Yu, Ronghua Yang,* Weihong Tan, Jingli Yuan “Design of room temperature phosphorescence-based molecular beacon for highly sensitive detection of nucleic acids in biological fluids”. Anal. Chem. 2011, 83, 1356–1362.

[39] Yinhui Li, Hao Wang, Jishan Li, Jing Zheng, Xinhua Xu, Ronghua Yang* “Simultaneous intracellular β-D-glucosidase and phosphodiesterase I activity measurements based on a triple-signaling fluorescent probe” Anal. Chem. 2011, 83, 1268–1274.  

[40] Xiangyuan Ouyang, Jianyu Jin, Jishan Li*, Ruqin Yu, Weihong Tan, Ronghua Yang,* Jingli Yuan “Conjugates of europium(III) complex and aptamer-wrapped carbon nanotubes: A new approach for label-free and time-resolved luminescent assay of protein” Anal. Chem. 2011, 83, 782-789.

[41] Jianbo Liu, Xiaohai Yang, Kemin Wang,* Ronghua Yang, Haining Ji, Liyan Yang, Chunlei Wu, “A switchable fluorescent quantum dot probe based on aggregation/disaggregation mechanism”, Chem. Commun. 2011, 47, 935–937.

[42] Hao Wang, Jishan Li, Yongxiang Wang,  Jiangyu Jin, Kemin Wang, Weihong Tan, Ronghua Yang* “Combination of DNA ligase reaction and gold nanoparticle-quenched fluorescent oligonucleotides: A simple and efficient approach for fluorescent assaying single-nucleotide polymorphisms” Anal. Chem. 2010,  82,  7684-7690  

[43] Yongxiang Wang, Jishan Li, Hao Wang, Jianyu Jin, Jinhua Liu, Kemin Wang, Weihong Tan, Ronghua Yang* “Silver ions-mediated conformational switch: Facile design of structure-controllable nucleic acid probes” Anal. Chem. 2010,  82,   6607-6612

[44] Na Shao, Hao Wang, Xiadi Gao, Ronghua Yang*, Winghong Chan* “Design spiropyran-based fluorescent anion probe and its application for urinary pyrophosphate detection” Anal. Chem. 2010,  82, 4628-4636

[45] Jing Zheng, Jishan Li,  Xiaoxia Gao, Jianyu Jin, Kemin Wang, Ronghua Yang* Weihong Tan “Modulating molecular level space proximity: A simple and efficient strategy to design structured DNA probes” Anal. Chem. 2010, 82, 3914-3921.

[46] Jishan Li, Ting Deng, Xia Chu*, Ronghua Yang*, Jianhui Jiang, Guoli Shen, and Ruqin Yu Rolling circle amplification combined with gold nanoparticle aggregates for highly sensitive identification of single-nucleotide polymorphisms” Anal. Chem. 2010, 82, 2811–2816.  

[47] Na Shao, Jianyu Jin, Hao Wang, Jing Zheng, Ronghua Yang*, Winghong Chan*, Zeper Abliz “Design of bis-spiropyran ligands as dipolar molecule receptors and application to in vivo glutathione fluorescent probes”  J. Am. Chem. Soc. 2010, 132,725-736

[48] Yongxiang Wang, Jishan Li, Jianyu Jin, Hao Wang, Hongxi Tang, Ronghua Yang,* Kemin Wang* “A new strategy for molecular beacon binding readout: Separating molecular recognition element and signal reporter”  Anal. Chem. 2009,  81, 9703-9709

[49] Xiaobing Bu, Huaping Chen, Hongwei Gai*, Ronghua Yang, Edward S Yeung “Scattering imaging of single quantum dots with dark-field microscopy. Anal. Chem. 2009, 81, 7507-7509.

[50] Hao Wang, Ronghua Yang*, Liu Yang, Weihong Tan* “Nucleic acid conjugated nanomaterials for enhanced molecular recognition (invited review)” ACS nano, 2009, 3, 2451-2460.

[51] Yue Liu, Yongxiang Wang, Jianyu Jin, Hao Wang, Ronghua Yang*, Weihong Tan* “Fluorescent assay of DNA hybridization with label-free molecular switch: Reducing background-signal and improving specificity by using carbon nanotubes ” Chem. Commun. 2009, 665-667.  

[52] Ronghua Yang*, Jianyu Jin, Liping Long, Yongxiang Wang, Hao Wang, and Weihong Tan*Reversible molecular switching of molecular beacon: Controlling DNA hybridization kinetics and thermodynamics using mercury (II) ions” Chem. Commun. 2009, 322-324  

[53] Hao Wang, Yongxiang Wang, Jianyu Jin, Ronghua Yang* Gold nanoparticle-based colorimetric and “turn-on” fluorescent probe for mercury (II) ions in aqueous solution” Anal. Chem. 2008, 80,  9021-9028.

[54] Yanrong Wu, Joseph A. Phillips, Haipeng Liu, Ronghua Yang, Weihong Tan* “Carbon nanotubes protect DNA strands during cellular delivery” ACS Nano 2008, 2, 2023-2028.

[55] Renfa Cui, Qing Li, Dustin E. Gross, Xin Meng, Bo Li, Manuel Marquez, Ronghua Yang,* Jonathan L. Sessler,* Yuanhua Shao* “Anion transfer at a micro-water/1,2-dichloroethane interface facilitated by β-octafluoro-meso-octamethylcalix[4]pyrrole” J. Am. Chem. Soc. 2008, 130,14364 – 14365.

[56] Ronghua Yang, Zhiwen Tang, Jilin Yan, Huaizhi Kang, Youngmi Kim, Zhi Zhu, Weihong Tan* “Noncovalent assembly of carbon nanotubes and single-stranded DNA: An effective sensing platform for probing biomoleular interactions” Anal. Chem. 2008, 80, 7408-7413.

[57] Zhiwen Tang, Prabodhika Mallikaratchy, Ronghua Yang,Youngmi Kim, Zhi Zhu, Hui Wang,  Weihong Tan* “Aptamer switch probe based on intramolecular signal transduction” J. Am. Chem. Soc. 2008, 130, 11268-11269.

[58] Zhi Zhu, Zhiwen Tang, Joseph A. Phillips, Ronghua Yang, Hui Wang, Weihong Tan* “Regulation of singlet oxygen generation using single-walled carbon nanotubes” J. Am. Chem. Soc. 2008, 130, 10856-10857.

[59] Ronghua Yang,* Jianyu Jin, Yan Chen, Na Shao, Zhiwen Tang, Yanrong Wu,  Zhi Zhou, Weihong Tan* “Carbon nanotube-quenched fluorescent oligonucleotide: probes that fluoresce upon hybridization” J. Am. Chem. Soc. 2008, 130, 8351-8358. 

[60] Na Shao, Jianyu Jin, Hao Wang, Ying Zhang, Ronghua Yang*, Winghong Chan* “Tunable photochromism of spirobenzopyran via selective metal ion coordination: An efficient visual and ratioing fluorescent probe for divalent copper ion” Anal. Chem. 2008, 80, 3466-3475.

[61] Na Shao, Jianyu Jin, Guilan Wang, Ying Zhang Ronghua Yang*, Jingli Yuan*Europium(III) complex-based luminescent sensing probes for multi-phosphate anions: modulating selectivity by ligand choice” Chem. Commun. 2008, 1127-1129.

[62] Na Shao, Jianyu Jin, Sinman Cheung, Ronghua Yang*, Winghong Chan* “A spiropyran-based ensemble for visual detection of cysteine and homocysteine in neutral aqueous solution” Angew. Chem. Int. Ed. 2006, 45, 4944-4948.  

[63] ShunYing Liu,  Lei Fang, Yongbing He,  Winghong Chan, Kaitai Yeung, Yuenkit Cheng,  Ronghua Yang “A cholic acid-based fluorescent sensor for dicarboxylates and acidic amino acids in aqueous solutions”  Org. Lett. 2005, 7, 5825-5828.

[64] Na Shao, Ying Zhang, Sinman Cheung, Ronghua Yang*, Winghong Chan, Tain Mo, Kean Li, Feng Liu “A copper ion-selective fluorescent sensor based on inner filter effect using a spiropyran derivative” Anal. Chem. 2005, 77, 7294-7303.

[65] Ying Zhang, Ronghua Yang*, Feng Liu, Ke’an Li, “Fluorescent sensor for imidazole derivatives based on monomer-dimer equilibrium of a zinc porphyrin complex in a polymeric film” Anal. Chem. 2004, 76, 7336-7345.

[66] Ronghua Yang, Ke’an Li, Feng Liu, Na Li, Fenglin Zhao, Winghong Chan “3, 3¢, 5, 5¢- Tetramethyl-N-(9-anthrylmethyl)benzidine: A dual-signaling fluorescent reagent for optical sensing of aliphatic aldehydes without interference of environment pH” Anal. Chem. 2003, 75, 3908-3914.

[67] Ronghua Yang, Winghong Chan, Albert W. M. Lee, Pingfang Xia, Hongkui Zhang, Ke’an Li “A ratiometric fluorescent sensor for Ag(I) with high selectivity and sensitivity” J. Am. Chem. Soc. 2003, 125, 2884-2885.

[68] Ronghua Yang, Ke’an Li*, Kemin Wang, Na Li, Fenglin Zhao, Feng Liu “Porphyrin assembly on b-cyclodextrin for selective sensing and detection of a zinc ion based on the dual emission fluorescence ratio” Anal. Chem. 2003, 75, 612-621.

[69] Ronghua Yang, Kemin Wang*, Liping Long, Dan Xiao, Xiaohai Yang. “A selective optode membrane for histidine based on fluorescence enhancement of meso-meso-linked porphyrin dimer ”. Anal. Chem., 2002, 74, 1088-1096.

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